ContentsPrefaceChapter1 Introduction 11.1 Conception of Enzyme and Its Development 21.2 The Characteristics of Enzyme Action 31.3 Factors that Affecting Enzyme Action 71.4 Classification and Nomenc1ature of Enzymes 111.5 Determination of Enzyme Activity 191.6 Methods for Enzymes Production 241.7 Development Survey of Enzyme Engineering 28Review and Thinking 32Chapter 2 Enzyme Production by Microbial Fermentation332.1 Regulation of Enzyme Biosynthesis in Microbial Cells 342.2 Characteristic of Microbes for Enzyme Production 492.3 Fermentation Process Conditions and Its Connol 532.4 Enzyme Fermentation Kinetics 652.5 Enzyme Production by Immobilized Cell Fermentation 712.6 Enzyme Production by Innnobilized Protoplast Fermentation 78Review and Thinking 80Chapter 3 Enzyme Production by Animal and Plant CelI Cultures 813.1 The Regulation of Enzyme Biosynthesis in Animal and Plant Cells 823.2 Enzyme Production by Plant Cell Culture 863.3 Enzyme Production by Animal Cell Culture 97Review and Thinking 105Chapter 4 Extraction， Separation and purification，tioo of Enzymes 1064.1 Cell Disruption 1064.2 Enzyme Extraction 1104.3 Precipitation Separation 1134.4 Centrifugal Separation 1184.5 Filtration and Menlbrane Separation 1244.6 Chrornatographic Separation 1304.7 Electrophoresis Separation 1504.8 Exnaction Separation 1604.9 Crystallization 1674.10 Concentration and Drying 170Review and Thinking 172Chapter 5 Enzyme Molecular Modification 1745.1 Metal Ion Substitution Modification 1755.2 Macromolecular Binding Modification 1775.3 Side Chain Group Modification 1815.4 Peptide Chain Limited Hydrolysis Modification 1875.5 Nucleotide Chain Cleavage Modification 1885.6 Amino Acid Substitution Modification 1895.7 Nucleotide Substitution Modification 1925.8 Physical Modification 1935.9 Application of Enzyme Molecular Modification 194Review and Thlnking 200Chapter 6 ImmobilizatiOll of Enzymes， cells and Protoplasts 2016.1 Enzyme Immobilization 2036.2 Cell Immo biliza tion 2166.3 Protoplast Immobilization 228Review and Thlnking 231Chapter 7 Enzyme Catalysis in Non-aqueous phases 2327.1 The Main Scopes of Enzyme Catalysis in Non-aqueous Phase 2337.2 Effects of Water and Organic Solvents on Enzyme Catalysis in Organic Media 2347.3 The Catalytic Characters of Enzymes in Organic Media 2407.4 Conditions of Enzyme Catalytic Reaction in Organic Media and Its Control 2447.5 Application of Enzyme Catalysis in Non-aqueous Phase 251Review and Thlnking 260Chapter 8 Enzyme Directed Evolotion 2618.1 The characteristics of Enzyme Directed Evolution 2638.2 Random Mutation of Enzyme Gene 2648.3 Directed Selection of Enzyme Gene Mutants 2708.4 The Applications of Enzyme Directed Evolution 281Review and Thlnking 283Chapter 9 Enzyme Reactors 2859.1 Types of Enzyme Reactors 2869.2 Choice of Enzyme Reactors 2929.3 Design of Enzyme Reactors 2969.4 Enzyme Reactor Operation 301Review and Thlnking 306Chapter 10 Application of Enzymes 30710.1 Enzymes Application in Pharmaceutical Industry 30710.2 Application of Enzymes in Food 32810.3 Enzyme Application in Light and Chemical Industries 34210.4 Enzyme Application in Environmental Protection 34910.5 Enzyme Application in Biotechnology 352Review and Thinking 358Main references 359
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